Comments
Polyacrylamide gels are formed by polymerization of acrylamide monomers to long chains and crosslinking of these chains with N,N'-methylenebisacrylamide (bisacrylamide). The concentration of acrylamide and the ratio of acrylamide to bisacrylamide determine the pore size of the gel. Bisacrylamide is more hydrophobic and therefore less water soluble than acrylamide. It is recommended to dissolve bisacrylamide in lukewarm water. All bisacrylamide solutions should be filtered, as is the case with acrylamide solutions.
Literature
(1) Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87. Electrophoresis in agarose and acrylamide gels. (2) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (3) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Greene Publishing & Wiley-Interscience, New York.
