Comments
Molybdate is a tyrosine phosphatase inhibitor. Its sodium or ammonium salts are added to the inhibitor cocktails in a working concentration ranging from 1 - 10 mM (e. g. ref. 3). Besides, molybdate is used as a reagent for the demonstration of inorganic phosphate, e. g. released by adenine triphosphatase (1).
Literature
(1) Lowry, O.H. (1957) Methods Enzymol. 4, 366-381Micromethods for the assay of enzymes. (2) Rivera, V.M. et al. (1993) Mol. Cell. Biol. 13, 6260-6273A growth factor-induced kinase phosphorylates the serum response factor at a site that regulates its DNA-binding activity. (3) Wu, Y.Y. & Bradshaw, R.A. (1993) J. Cell Biol. 121, 409-422. Effect of nerve growth factor and fibroblast growth factor on PC12 cells: Inhibition by Orthovanadate.