Comments
A commonly used method is the "guanidinium thiocyanate-phenol-chloroform" extraction. It is not essential to use phenol or chloroform if the extraction is for a northern blot or southern blot, since gel electrophoresis followed by transfer to a membrane separates the RNA/DNA from the proteins. Since these methods (northern or Southern blot) use labeled oligonucleotide probes, peptides that manage to pass through the process are generally not of concern unless the peptide has RNase or DNase activity or and only if an enzyme manages to renature, which should not happen if the appropriate protocols are followed correctly. A possible exception may arise from work with hyperthermophilic extremophiles because some enzymes from these organisms may remain stable under unusual circumstances.
FAQs
What is the CAS number of Cesium Chloride?
The CAS number of Cesium Chloride is 7647-17-8.CAS Cesium Chloride?
The CAS number of Cesium Chloride is 7647-17-8.CAS 7647-17-8?
The CAS number 7647-17-8 is assigned to Cesium Chloride. What does Cesium Chloride 99.9% mean?
Cesium Chloride 99.9% means that the mass fraction is 0,999. In other words, in a sample of 100 g Cesium Chloride 99.9% the mass of 99,9 g Cesium Chloride is present.What is the purity of Cesium Chloride?
The purity of Cesium Chloride is 99,9%.What does Cesium Chloride 99.9% mean?
Cesium Chloride 99.9% means that the mass fraction is 0,999. In other words, in a sample of 100 g Cesium Chloride 99.9% the mass of 99,9 g Cesium Chloride is present.What is the purity of Cesium Chloride?
The purity of Cesium Chloride is 99,9%.
Literature
(1) Miller, H. (1987) Methods Enzymol. 152, 145-179. Practical aspects on the preparation of phage and plasmid DNA. (2) Dorin, M. & Bornecque, C.A. (1995) BioTechniques 18, 90-92. Rapid separation of plasmid DNA by discontinuous gradient centrifugation. (3) Hildeman, D.A. & Muller, D. (1997) BioTechniques 22, 878-879. Improved plasmid DNA yields by removal of CsCl during ethanol precipitation.
