Comments
Polyvinylpyrolidone (PVP) with a molecular weight of 10,000 is suitable in combination with high salt concentrations (3.75 M ammonium acetate) for precipitation of DNA from plant extracts. The working solution is prepared fresh before use (final concentration 6% v/v; ref. 1) or stored as a 20% stock solution at -20°C (final concentration 6% v/v; ref. 2).
Literature
(1) Kim, C.S. et al. (1997) Nucleic Acids Res. 25, 1085-1086. Isolation of genomic DNA from fruit trees and conifers. (2) Fu, R.-Z. et al. (1998) BioTechniques 25, 796-801. Isolation of genomic DNA from dried plant rhizomes / roots.
