Comments
Ficoll® 400 is a component of Denhardt's solution and several gel loading buffers (2 [Page 6.12]). The addition of Ficoll® 400, sucrose or glycerol increase the density of the loading buffers and facilitate the loading of the sample onto the gel. Composition of loading buffers: I. 0.25 % Bromophenol blue, 0.25 % xylene cyanol FF, 15 % Ficoll® 400 in water (store at room temperature)(according to ref. 2) II. Alkaline loading buffer 300 mM NaOH, 6 mM EDTA, 18 % Ficoll® 400, 0.15 % Bromocresol green, 0.25 % xylene cyanol FF in water (store at +4°C)
Literature
(1) Denhardt, D.T. (1966) Biochem. Biophys. Res. Com. 23, 641-646. A membrane-filter technique for the detection of complementary DNA. (2) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition; p. 9.47-9.55, 6.12. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
