Comments
Guanidinium thiocyanate is also used to lyse cells and virus particles to extract RNA and DNA, and where, in addition to its lysing action by denaturing, it prevents the activity of RNase and DNase enzymes that could damage the extract.
Literature
(1) Johnson, D.A. et al. (1987) Gene Anal. Techn. 1, 3-8. Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. (2) Harlow, E., Lane, D. (eds.) (1988) Antibodies A Laboratory Manual; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (3) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (4) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Page 2.10.16 (Suppl. 42) Greene Publishing & Wiley-Interscience, New York.
