Comments
For most applications in the electrophoresis of nucleic acids or proteins, polyacrylamide gels are prepared from 30 % or 40 % stock solutions with a ratio Acrylamide : Bisacrylamide of 29 : 1 or 37.5 : 1.
Literature
(1) Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87. Electrophoresis in agarose and acrylamide gels. (2) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (3) Hames, B.D. (1990) Chapter 3 in Gel Electrophoresis of Proteins: A Practical Approach 2nd ed. (Hames, B.D. & Rickwood, D. eds.) IRL Press. (4) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Greene Publishing & Wiley-Interscience, New York.
