Comments
DNA-Dye NonTox is a dye for the staining of DNA in gel electrophoresis. It is as sensitive as ethidium bromide, but lacking the negative properties of ethidium bromide: it is non-hazardous, non-mutagenic and possesses a low toxicity (LC > 5000 mg/kg). Besides, DNA-Dye NonTox complies to the Clean Water Act due to its very low environmental impact. There is no risk of water pollution, as compared to ethidium bromide and there are no special measures to be taken with respect to the waste management. The integritiy and structure of DNA won't be affected by staining with DNA-Dye NonTox. Therefore, significantly higher transformation rates are achieved, as compared to ethidium bromide-stained plasmid DNA.DNA-Dye NonTox is supplied as a ready-to-use 6X loading dye, containing three tracking dyes: bromophenol blue, xylene cyanol and orange G. Since the loading dye is added directly to the sample, there is only a very low background staining observed. After the gel run, no destaining is required.DNA-Dye NonTox attaches to DNA strands, but does not intercalate. Due to this mechanism, variations in the migration behaviour between samples and markers were rarely observed. Bands with very low DNA amounts (5-10 ng/band) may migrate slowlier by up to 10 ± 2% than the usually higher concentrated size marker bands.
