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LB-Agar - Powder according to Lennox

Code
A7459

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Box prices only valid with purchase of full box.

Packs sizes (1)

Code packaging size price per unit Quantity Price Per Box Quantity
A7459,1000KG
Code
A7459,1000KG
 packaging size
1 kg
 price per unit
192,80€
quantity
Physical Description:
Solid
Product Code:
A7459
Product Name:
LB-Agar - Powder according to Lennox
Short Description:
usage: for Luria-Bertani Agar plates according to Lennox for cultivation of E. coli in molecular biology
Specifications:
Appearance after heating: clear, brown liquid
Gel strength: passes test
Composition:
Agar: 15 g/L
Yeast extract: 5 g/L
NaCl: 5 g/L
Tryptone: 10 g/L
WGK:
1
Storage:
RT
CS:
38210000
Download TDS file for complete specifications

Comments

The packaging sizes differ in terms of the amount: you can choose between packages in liter (L) or gram (g) and kilogram (kg), respectively. Liter packs dissolved in the corresponding volume will give the correct concentration. When using the gram and kilogram packs, aliquots of the powder have to be dissolved. To prepare one liter of LB-Agar according to Lennox, 35 g of powder are dissolved.Directions to prepare 1 liter medium/agar: Dissolve the powder in 900 ml of water; adjust pH to 7.0 with 5 N NaOH (approx. 0.2 ml), fill to 1000 ml and autoclave. Antibiotics and nutritional supplements should be added only after the solution has cooled to 50°C or below, sine many of them are heat-sensitive.

Literature

(1) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Page 1.1.2 (Suppl. 59) Greene Publishing & Wiley-Interscience, New York.