Comments
Sorbitol is used in high concentration for cell disruption. The stabilizing buffer for enzymatic lysis of yeast contains 1 M Sorbitol (1). Concentrations of 300 mM and 400 mM, respectively, are used for the extraction of chloroplasts and thylacoids, respectively (2).
Literature
(1) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition; Page 18.36, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (2) Gegenheimer, P. (1990) Methods Enzymol. 182, 174-193. Preparation of extracts from plants.