Comments
Tetracycline was prepared first syntheticaly from chlorotetracycline, later it was isolated from Streptomyces viridifaciens. Tetracycline is active against gram positive and gram negative cocci and bacteria. The introduction of the tetracycline-controlled gene expression system brought a new application for the antibiotic molecular biology (2-4).Stability: Tetracycline hydrochloride is stable if stored in a dry place and protected from light. It is water-soluble with approx. 11 mg/ml at 28°C. The stability in solutions is optimal at pH 3 - 5.2 (shelf life approx. 6 - 12 days). It may be stored between -20°C and 37°C. Since Tetracyclin is light-sensitive, solutions and agar plates containing TC should be protected from light. The recommended working concentration is 10 - 50 μg/ml, the stock solution e.g. 1.25 mg/ml. Magnesium ions antagonize with the activity of TC.
Literature
(1) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (2) Gossen, M. & Bujard, H. (1992) Proc. Natl. Acad. Sci. USA 89, 5547-5551. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. (3) Gossen, M. et al. (1995) Science 268, 1766-1769. Transcriptional activation by Tetracyclines in mammalian cells. (4) Yin, D.X. et al. (1996) Anal. Biochem. 235, 195-201. Tetracycline-controlled gene expression system achieves high-level and quantitative control of gene expression. (5) Freundlieb, S. et al. (1997) Methods Enzymol. 283, 159-173. Tetracyclin-controlled gene expression systems for investigations of the cell cycle.
