Comments
Cytochalasin B inhibits, like Cytochalasin A, not just the actin and tubulin filament formation, but the glucose uptake into cells as well. Therefore, if you study the microfilament system, it may be advantageous to employ Cytochalasin D instead, since this drug does not influence glucose uptake. Stability and Solubility: Cytochalasin B dissolved in DMSO shows no decrease in potency even after 3 years if stored at +4°C. In DMF, Cytochalasin B shows the highest solubility with 492 mg/ml. In DMSO 371 mg/ml can be dissolved, while in ethanol solubility drops to 35.4 and in Aceton to 10.3 mg/ml, respectively (Merck Index 13th Edition 2001; page 489, monograph 2819). Please note the toxicity of certain solvents. The problem can be circumvented if stock solutions are prepared in high concentrations (100X or even higher e.g. 10 mM) and the solvent becomes diluted if added to the sample. The working concentration is 1 to 20 μM.
